The MemoGene™ Solution bypasses current biotechnological barriers for precise genome editing and offers 3 main consumer applications in 2 validation phases:
Phase 1 – DNA Deletion:
Viral-based targeted deletion of DNA is an extremely useful tool for the development and breeding of new plant varieties, especially in light of general genomic advances and the method's independence of tissue culture.
a) Targeted ID-ing:
Via the deletion of readily identifiable segments of non-coding DNA (nuclear or plastid), a plant can be easily “tagged” for patentability. There is currently no efficient way of avoiding infringement or of protecting parent lines. The ability to “mark” plant varieties is expected to be of enormous benefit to breeders by reducing losses in royalty and income, as well as the need for legal processes and their associated high cost. The ID-ing will not change the plant's traits, it will not depend on tissue culture, it will be unique, and the outcome will allow the introduction of tagged plants to new foreign markets as well as technology transfer within the industry.
b) Targeted Mutagenesis:
Expression or suppression of specific plant traits will be achieved through deletion or alteration of gene (nuclear or plastid) activity, creating new commercially desirable “tailor-made” varieties.
Phase 2 – DNA Insertion/Substitution:
Targeted Genetic Modification:
The new viral-based method of genome modification will be used to create new characteristics in plants through site-specific substitution/insertion of relevant DNA sequences in nuclear and plastid genomes. While this may involve a longer time to market than mutagenesis, the long-term commercial possibilities here are enormous. Key advantages of MemoGene™ over the common genetic engineering methods are: a generic process which requires only small modifications for use in different plants (no need for a highly specific protocol for each crop or variety); no need for tissue culture steps; possibility of modifying traits controlled by DNA that is present in the nucleus and cell plastids (mitochondria and chloroplasts); applicable even in recalcitrant plants that have proven resistant to genetic engineering using other methods.